Lanes 1 and 2). There are data (from ongoing lab studies) that indicate some lobsters, post-sperm sampling, still extrude fertilized clutches of eggs (n=3) and are capable of fertilizing more than one clutch of eggs using the same spermatophore. A noninvasive method for. In the laboratory, pre-molt females will be housed with males of different sizes to determine if small males are capable of mating with larger females. However, several recent findings suggest that the full reproductive potential of a portion of the sexually mature females is not being met, possibly due to fishery induced skewed sex ratios and a paucity of large males.
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Lavalli sperm limitation. The working hypothesis was that some lobsters were carrying eggs that had not been fertilized and, as a result, ‘fell off’. The overarching theme of this project is to determine if sperm limitation is becoming a factor in specific regions of the american lobster fishery and, if so, what are some of the implications for lobster populations and the fishery that relies upon them. ) and the netherlands organization for scientific research (nwo). Third, the very strong binding of biotin to avidin/streptavidin offers another advantage in that it allows increasingly higher stringencies to be used during purification without fear of early elution of the tagged protein. To these ends, we coexpressed bacterial bira biotin ligase and hematopoietic transcription factors tagged by an n-terminal fusion of a small artificial peptide previously shown to be biotinylated by bira (. In conclusion, the efficient biotinylation of specific proteins in mammalian cells demonstrated here raises the prospect of applying the advantages and flexibility of the well-developed biotin/avidin technology in the single-step purification and characterization of mammalian protein complexes.
, an obligate reef-dweller
Biotin/avidin binding is the strongest noncovalent interaction known in nature (. Once we have an adequate understanding of this important subject, this knowledge can be used to more effectively manage this economically and biologically influential marine resource.